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Sample description
Sample ID: 1100
Anatomical region: head
Organ system: eye
Term: optic II nerve
Sub-term: optic II nerve
Sampling: 2017-04-11 / provided by Lohi lab
Transport media: RNAlater
Additional info:
From subject: RW356
Breed (variant): Rottweiler
Sex: Male
Age: 6y 2m
Steril./Castr.: No
Reports:  Necropsy report   Clinical test results
WGS ID: RW356A1, BioProject PRJNA907518, SRR22520747
Additional info:
Study: Study of gene expression across dog tissues
Description: This project will generate the critical resources to better utilize various canine models to advance both dog and human health.
Status / Visibility: Submitted / Public
Publication: The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728)
Other IDs: PRJNA907518
Additional info:
 
Sample processing
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
520 2017-09-12 RW356 1100 (5.12) 155.0 (QUBIT) 19 mg 30 4650.0 QIAZOL + QIAGEN RNeasy Mini Kit 8.10 1.876 1.239
 
Applied assays   
STRT2-seq
 
Technical replicate [1218]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP8_43 Illumina NextSeq 550 No Aug. 16, 2018 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP8_43  STRT2_FP8_43_canine_mapped.bam STRT2_FP8_43_canine_mapped.fq.gz CanFam3.1 3.0
STRT2_FP8_43  STRT2_FP8_43_canine_mapped.fq.gz CanFam3.1 3.0
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP8_43 21,669 450,174 65.20
 
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
176 2017-04-26 RW356 1100 70.0 (QUBIT) 20 mg 30 2100.0 QIAGEN RNeasy Mini Kit 7.20 1.995 1.881
 
Applied assays   
STRT2-seq
 
Technical replicate [1041]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP3_47 Illumina NextSeq 550 No Dec. 12, 2018 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP3_47  STRT2_FP3_47_canine_mapped.bam STRT2_FP3_47_canine_mapped.fq.gz CanFam3.1 3.0
STRT2_FP3_47  STRT2_FP3_47_canine_mapped.fq.gz CanFam3.1 3.0
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP3_47 170,739 2,615,683 72.80