Technical replicate [923]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP1_5	Illumina	NextSeq 550	No	April 19, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP1_5  STRT2_FP1_5_canine_mapped.fq.gz — CanFam3.1 — 3.0 — STRT2_FP1_5  STRT2_FP1_5_canine_mapped.bam STRT2_FP1_5_canine_mapped.fq.gz CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP1_5 — — 170,875 — 1,441,698 61.90 — —

Technical replicate [1335]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP11_5	Illumina	NextSeq 550	No	Oct. 25, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP11_5  STRT2_FP11_5_canine_mapped.bam STRT2_FP11_5_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP11_5  STRT2_FP11_5_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP11_5 — — 170,323 — 3,073,228 77.40 — —

Technical replicate [988]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP2_29	Illumina	NextSeq 550	No	Nov. 27, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP2_29  STRT2_FP2_29_canine_mapped.bam STRT2_FP2_29_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP2_29  STRT2_FP2_29_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP2_29 — — 112,426 — 2,577,634 69.00 — —