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Sample description
Sample ID: 1357
Anatomical region: forelimb
Organ system: hemolymphoid system
Term: bone marrow
Sub-term: bone marrow
Sampling: 2017-04-11 / provided by Lohi lab
Transport media: RNAlater
Additional info:
From subject: RW356
Breed (variant): Rottweiler
Sex: Male
Age: 6y 2m
Steril./Castr.: No
Reports:  Necropsy report   Clinical test results
WGS ID: RW356A1, BioProject PRJNA907518, SRR22520747
Additional info:
Study: Study of gene expression across dog tissues
Description: This project will generate the critical resources to better utilize various canine models to advance both dog and human health.
Status / Visibility: Submitted / Public
Publication: The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728)
Other IDs: PRJNA907518
Additional info:
 
Sample processing
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
275 2017-05-02 RW356 1357 200.0 (ND) 11 mg 30 6000.0 QIAGEN RNeasy Mini Kit 9.90 2.067 1.166 tissue samples used 1357 1359
 
Applied assays   
STRT2-seq
 
Technical replicate [1141]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP6_10 Illumina NextSeq 550 No June 15, 2018 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP6_10  STRT2_FP6_10_canine_mapped.bam STRT2_FP6_10_canine_mapped.fq.gz CanFam3.1 3.0
STRT2_FP6_10  STRT2_FP6_10_canine_mapped.fq.gz CanFam3.1 3.0
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP6_10 96,837 904,890 54.50
Technical replicate [1042]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP3_33 Illumina NextSeq 550 No Dec. 12, 2018 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP3_33  STRT2_FP3_33_canine_mapped.bam STRT2_FP3_33_canine_mapped.fq.gz CanFam3.1 3.0
STRT2_FP3_33  STRT2_FP3_33_canine_mapped.fq.gz CanFam3.1 3.0
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP3_33 310,817 4,099,033 70.10
Technical replicate [1379]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP6_41 Illumina NextSeq 550 No June 15, 2018 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP6_41  STRT2_FP6_41_canine_mapped.bam STRT2_FP6_41_canine_mapped.fq.gz CanFam3.1 3.0
STRT2_FP6_41  STRT2_FP6_41_canine_mapped.fq.gz CanFam3.1 3.0
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP6_41 1,625 30,884 19.20