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Sample description
Sample ID: 1414
Anatomical region: head
Organ system: central nervous system
Term: caudate nucleus
Sub-term: caudate nucleus
Sampling: 2016-09-20 / provided by Lohi lab
Transport media: RNAlater
Additional info: This was originally a training dog.
From subject: BC1642
Breed (variant): Border Collie
Sex: Male
Age: 6y 11m
Steril./Castr.: Not specified
Reports:  Necropsy report 
WGS ID: BC1642A1, BioProject PRJNA907518, SRR22520746
Additional info: This was originally a training dog. Epilepsy (cortex, hippocampus) + degenerative changes in large brain (mild neuronal loss, prominent capilaries, lipofuscinosis).
Study: Study of gene expression across dog tissues
Description: This project will generate the critical resources to better utilize various canine models to advance both dog and human health.
Status / Visibility: Submitted / Public
Publication: The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728)
Other IDs: PRJNA907518
Additional info: —
 
Sample processing
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
286 2017-05-30 BC1642 1414 115.0 (QUBIT) 22 mg 30 3450.0 QIAGEN RNeasy Mini Kit 9.10 — — nucleus caudatus tube used
 
Applied assays   
This sample was not sequenced yet.
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
706 2017-05-18 BC1642 CN (TS12.2.2020) 65.0 (ND) 15 mg 30 1950.0 QIAGEN RNeasy Mini Kit 8.60 2.138 2.395 —
 
Applied assays   
STRT2-seq
 
Technical replicate [1266]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP9_3 Illumina NextSeq 550 No Oct. 12, 2018 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP9_3  STRT2_FP9_3_canine_mapped.bam STRT2_FP9_3_canine_mapped.fq.gz CanFam3.1 — 3.0 —
STRT2_FP9_3  STRT2_FP9_3_canine_mapped.fq.gz — CanFam3.1 — 3.0 —
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP9_3 — — 234,638 — 1,573,560 66.90 — —