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Sample description
Sample ID: 1438
Anatomical region: head
Organ system: central nervous system
Term: thalamus
Sub-term: nucleus reuniens
Sampling: 2016-09-20 / provided by Lohi lab
Transport media: RNAlater
Additional info: This was originally a training dog.
From subject: BC1642
Breed (variant): Border Collie
Sex: Male
Age: 6y 11m
Steril./Castr.: Not specified
Reports:  Necropsy report 
WGS ID: BC1642A1, BioProject PRJNA907518, SRR22520746
Additional info: This was originally a training dog. Epilepsy (cortex, hippocampus) + degenerative changes in large brain (mild neuronal loss, prominent capilaries, lipofuscinosis).
Study: Study of gene expression across dog tissues
Description: This project will generate the critical resources to better utilize various canine models to advance both dog and human health.
Status / Visibility: Submitted / Public
Publication: The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728)
Other IDs: PRJNA907518
Additional info:
 
Sample processing
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
304 2017-08-16 1438 (15.11.2018) 135.0 (ND) 20 mg 30 4050.0 QIAGEN RNeasy Mini Kit 9.50 2.028 2.003
 
Applied assays   
CAGE-seq
 
Technical replicate [1813]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
CAGE_L_00206.BC12 Illumina HiSeq 2500 No Sept. 23, 2020 SR 1x50bp 50 bp HiSeq SR Rapid Cluster Kit v2 GD-402-4002 + HiSeq Rapid Duo cBot Sample Loading Kit CT-403-2001 + SBS Clinical Genomics Stockholm None
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
CAGE_L_00206.BC12  CAGE_L_00206.BC12.sorted.tar.gz CAGE_L_00206.BC12_qfilter_trimBC.fq.gz CanFam4 4
CAGE_L_00206.BC12  CAGE_L_00206.BC12_qfilter_trimBC.fq.gz 4
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
CAGE_L_00206.BC12 Nucleus reuniens - LGN pool 111,573,436 5,737,455 66.80
 
STRT2-seq
 
Technical replicate [1264]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP9_9 Illumina NextSeq 550 No Oct. 12, 2018 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP9_9  STRT2_FP9_9_canine_mapped.bam STRT2_FP9_9_canine_mapped.fq.gz CanFam3.1 3.0
STRT2_FP9_9  STRT2_FP9_9_canine_mapped.fq.gz CanFam3.1 3.0
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP9_9 359,315 1,875,172 59.80