| Sample ID: | 1466 |
|---|---|
| Anatomical region: | neck |
| Organ system: | central nervous system |
| Term: | spinal cord |
| Sub-term: | spinal cord cervical segment |
| Sampling: | 2016-09-20 / provided by Lohi lab |
| Transport media: | RNAlater |
| Additional info: | This was originally a training dog. |
| From subject: | BC1642 |
|---|---|
| Breed (variant): | Border Collie |
| Sex: | Male |
| Age: | 6y 11m |
| Steril./Castr.: | Not specified |
| Reports: | Necropsy report |
| WGS ID: | BC1642A1, BioProject PRJNA907518, SRR22520746 |
| Additional info: | This was originally a training dog. Epilepsy (cortex, hippocampus) + degenerative changes in large brain (mild neuronal loss, prominent capilaries, lipofuscinosis). |
| Study: | Study of gene expression across dog tissues |
|---|---|
| Description: | This project will generate the critical resources to better utilize various canine models to advance both dog and human health. |
| Status / Visibility: | Submitted / Public |
| Publication: | The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728) |
| Other IDs: | PRJNA907518 |
| Additional info: | — |
| RNA extraction | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| ID | Date | Bioanalizer ID | Stock Conc. (ng/µl) |
Tissue ammount | Sample volume | RNA amount (ng) |
Extraction method | RIN | Ratio A260/A280 |
Ratio A260/A230 |
Comments |
| 478 | 2016-10-31 | 9 | 144 (ND) | 60 mg | 50 | 7200 | QIAZOL + QIAGEN RNeasy Mini Kit | 8.70 | — | — | — |
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Applied assays
This sample was not sequenced yet.
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| RNA extraction | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| ID | Date | Bioanalizer ID | Stock Conc. (ng/µl) |
Tissue ammount | Sample volume | RNA amount (ng) |
Extraction method | RIN | Ratio A260/A280 |
Ratio A260/A230 |
Comments | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 712 | 2017-01-31 | BC1642 1466 (22.11) | 110 (QUBIT) | 17 mg | 30 | 4650 | QIAZOL + QIAGEN RNeasy Mini Kit | 8.60 | 2.091 | 1.807 | — | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Applied assays
STRT2-seq
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