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Sample description
Sample ID: 1555
Anatomical region: neck
Organ system: endocrine system
Term: thyroid gland
Sub-term: thyroid gland
Sampling: 2016-09-20 / provided by Lohi lab
Transport media: RLT buffer
Additional info: This was originally a training dog.
From subject: BC1642
Breed (variant): Border Collie
Sex: Male
Age: 6y 11m
Steril./Castr.: Not specified
Reports:  Necropsy report 
WGS ID: BC1642A1, BioProject PRJNA907518, SRR22520746
Additional info: This was originally a training dog. Epilepsy (cortex, hippocampus) + degenerative changes in large brain (mild neuronal loss, prominent capilaries, lipofuscinosis).
Study: Study of gene expression across dog tissues
Description: This project will generate the critical resources to better utilize various canine models to advance both dog and human health.
Status / Visibility: Submitted / Public
Publication: The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728)
Other IDs: PRJNA907518
Additional info:
 
Sample processing
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
727 2017-10-18 370 (ND) 20 mg 60 22200 Not specified 6.10 2.075 2.115
 
Applied assays   
STRT2-seq
 
Technical replicate [1554]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP14_27 Illumina NextSeq 550 No May 2, 2019 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP14_27  SDID_001937_STRT2_FP14_27_canine_mapped.bam SDID_001936_STRT2_FP14_27_canine_mapped.fq.gz CanFam3.1 3.0
STRT2_FP14_27  SDID_001936_STRT2_FP14_27_canine_mapped.fq.gz CanFam3.1 3.0
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP14_27 119,567 3,482,227 67.30
Technical replicate [1404]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP7_27 Illumina NextSeq 550 No Aug. 16, 2018 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP7_27  STRT2_FP7_27_canine_mapped.bam STRT2_FP7_27_canine_mapped.fq.gz CanFam3.1 3.0
STRT2_FP7_27  STRT2_FP7_27_canine_mapped.fq.gz CanFam3.1 3.0
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP7_27 77,248 1,680,323 69.30
 
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
459 2017-12-29 1555 (23.1.2018) 500 (QUBIT) 2 mm3 25 8775 Not specified 9.20 2.03 2.07
 
Applied assays   
This sample was not sequenced yet.