Technical replicate [975]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP2_16	Illumina	NextSeq 550	No	Nov. 27, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP2_16  STRT2_FP2_16_canine_mapped.bam STRT2_FP2_16_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP2_16  STRT2_FP2_16_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP2_16 — — 119,198 — 2,660,553 74.30 — —

Technical replicate [1370]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP2_42	Illumina	NextSeq 550	No	Nov. 27, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP2_42  STRT2_FP2_42_canine_mapped.bam STRT2_FP2_42_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP2_42  STRT2_FP2_42_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP2_42 — — 114,515 — 2,788,229 73.50 — —

Technical replicate [1233]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP8_8	Illumina	NextSeq 550	No	Aug. 16, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP8_8  STRT2_FP8_8_canine_mapped.bam STRT2_FP8_8_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP8_8  STRT2_FP8_8_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP8_8 — — 33,697 — 729,437 70.70 — —