Technical replicate [99]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
CAGE_L_00119.BC4	Illumina	HiSeq 2500	No	June 26, 2018	SR 1x50bp	50 bp	Hi-Seq High Output v4	Daub lab	Different % of spike-in (8, 10, 12, 14, 16, 18, 20, 22% in each lane) for CAGE set 03
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments CAGE_L_00119.BC4  CAGE_L_00119.BC04_qfilter_trimBC.fq.gz — CanFam4 — 4 — CAGE_L_00119.BC4  CAGE_L_00119.BC04.sorted.tar.gz CAGE_L_00119.BC04_qfilter_trimBC.fq.gz CanFam4 — 4 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments CAGE_L_00119 Heart muscle pool 23,713,706 — — 18,643,940 78.62 18.75 —

Technical replicate [1031]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP3_12	Illumina	NextSeq 550	No	Dec. 12, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP3_12  STRT2_FP3_12_canine_mapped.bam STRT2_FP3_12_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP3_12  STRT2_FP3_12_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP3_12 — — 239,302 — 6,843,523 72.80 — —