Technical replicate [1085]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP4_28	Illumina	NextSeq 550	No	May 14, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP4_28  STRT2_FP4_28_canine_mapped.bam STRT2_FP4_28_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP4_28  STRT2_FP4_28_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP4_28 — — 102,292 — 699,127 67.20 — —

Technical replicate [1419]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP2_20	Illumina	NextSeq 550	No	Nov. 27, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP2_20  STRT2_FP2_20_canine_mapped.bam STRT2_FP2_20_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP2_20  STRT2_FP2_20_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP2_20 — — 138,952 — 4,263,408 71.60 — —

Technical replicate [1372]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP4_44	Illumina	NextSeq 550	No	May 14, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP4_44  STRT2_FP4_44_canine_mapped.bam STRT2_FP4_44_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP4_44  STRT2_FP4_44_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP4_44 — — 135,670 — 1,023,002 67.90 — —