Technical replicate [1251]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP9_24	Illumina	NextSeq 550	No	Oct. 12, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP9_24  STRT2_FP9_24_canine_mapped.bam STRT2_FP9_24_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP9_24  STRT2_FP9_24_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP9_24 — — 366,409 — 4,563,309 71.00 — —

Technical replicate [1075]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP4_41	Illumina	NextSeq 550	No	May 14, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP4_41  STRT2_FP4_41_canine_mapped.bam STRT2_FP4_41_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP4_41  STRT2_FP4_41_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP4_41 — — 140,251 — 1,553,604 71.50 — —

Technical replicate [1386]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP9_33	Illumina	NextSeq 550	No	Oct. 12, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP9_33  STRT2_FP9_33_canine_mapped.bam STRT2_FP9_33_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP9_33  STRT2_FP9_33_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP9_33 — — 304,196 — 3,535,248 71.50 — —