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Sample description
Sample ID: 3359
Anatomical region: abdominal cavity
Organ system: alimentary system
Term: pancreas
Sub-term: pancreas left lobe
Sampling: 2017-09-12 / provided by Lohi lab
Transport media: RNAlater
Additional info:
From subject: RW589
Breed (variant): Rottweiler
Sex: Male
Age: 5y 1m
Steril./Castr.: No
Reports:  Necropsy report   Clinical test results
WGS ID: RW589A1, BioProject PRJNA907518, SRR22520744
Additional info:
Study: Study of gene expression across dog tissues
Description: This project will generate the critical resources to better utilize various canine models to advance both dog and human health.
Status / Visibility: Submitted / Public
Publication: The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728)
Other IDs: PRJNA907518
Additional info:
 
Sample processing
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
509 2017-12-05 RW589 3359 1655.0 (ND) 18 mg 100 165500.0 QIAGEN RNeasy Mini Kit 8.40 2.168 2.216
 
Applied assays   
CAGE-seq
 
Technical replicate [1903]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
CAGE_L_00214.BC15 Illumina HiSeq 2500 No Nov. 6, 2020 SR 1x50bp 50 bp HiSeq SR Rapid Cluster Kit v2 GD-402-4002 + HiSeq Rapid Duo cBot Sample Loading Kit CT-403-2001 + SBS Clinical Genomics Stockholm
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
CAGE_L_00214.BC15  CAGE_L_00214.BC15_qfilter_trimBC.fq.gz 4
CAGE_L_00214.BC15  CAGE_L_00214.BC15.sorted.tar.gz CAGE_L_00214.BC15_qfilter_trimBC.fq.gz CanFam4 4
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
CAGE_L_00214.BC15 Pancreas left lobe only 73,640,682 4,831,480 83.99