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Sample description
Sample ID: 3828
Anatomical region: abdominal cavity
Organ system: alimentary system
Term: jejunum
Sub-term: jejunum (mucosa)
Sampling: 2017-11-01 / provided by Lohi lab
Transport media: Liquid nitrogen
Additional info:
From subject: AM398
Breed (variant): Alaskan Malamute
Sex: Female
Age: 7y 1m
Steril./Castr.: No
Reports:  Necropsy report 
WGS ID:
Additional info:
Study: Study of gene expression across dog tissues
Description: This project will generate the critical resources to better utilize various canine models to advance both dog and human health.
Status / Visibility: Submitted / Public
Publication: The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728)
Other IDs: PRJNA907518
Additional info:
 
Sample processing
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
881 2019-11-20 3828 (26.11.2019) 524.0 (ND) 2 mm3 300 157485.0 QIAZOL 7.00 1.92 0.96
 
Applied assays   
STRT2-seq
 
Technical replicate [1713]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP17_29 Illumina NextSeq 550 No Jan. 27, 2020 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP17_29  SDID_002254_STRT2_FP17_29_canine_mapped.bam SDID_002253_STRT2_FP17_29_canine_mapped.fq.gz CanFam3.1 3.0
STRT2_FP17_29  SDID_002253_STRT2_FP17_29_canine_mapped.fq.gz CanFam3.1 3.0
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP17_29 6,943,544 68.10