You are logged in as AnonymousUser


 
Sample description
Sample ID: 4088
Anatomical region: head
Organ system: eye
Term: cornea
Sub-term: cornea
Sampling: 2017-11-28 / provided by Lohi lab
Transport media: RNAlater
Additional info:
From subject: CLK294
Breed (variant): Collie( smooth)
Sex: Male
Age: 4y 6m
Steril./Castr.: No
Reports:  Necropsy report   Clinical test results
WGS ID:
Additional info:
Study: Study of gene expression across dog tissues
Description: This project will generate the critical resources to better utilize various canine models to advance both dog and human health.
Status / Visibility: Submitted / Public
Publication: The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728)
Other IDs: PRJNA907518
Additional info:
 
Sample processing
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
855 2019-11-08 4088 (21.11.2019) 168.0 (ND) 2 mm3 25 4207.0 QIAZOL + QIAGEN RNeasy Mini Kit 9.20 1.99 0.73
 
Applied assays   
STRT2-seq
 
Technical replicate [1684]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP16_10 Illumina NextSeq 550 No Dec. 23, 2019 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP16_10  SDID_002085_STRT2_FP16_10_canine_mapped.fq.gz CanFam3.1 3.0
STRT2_FP16_10  SDID_002086_STRT2_FP16_10_canine_mapped.bam SDID_002085_STRT2_FP16_10_canine_mapped.fq.gz CanFam3.1 3.0
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP16_10 3,522,399 70.70