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Sample description
Sample ID: 4137
Anatomical region: neck
Organ system: endocrine system
Term: parathyroid gland
Sub-term: parathyroid gland
Sampling: 2017-11-28 / provided by Lohi lab
Transport media: RNAlater
Additional info:
From subject: CLK294
Breed (variant): Collie( smooth)
Sex: Male
Age: 4y 6m
Steril./Castr.: No
Reports:  Necropsy report   Clinical test results
WGS ID:
Additional info:
Study: Study of gene expression across dog tissues
Description: This project will generate the critical resources to better utilize various canine models to advance both dog and human health.
Status / Visibility: Submitted / Public
Publication: The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728)
Other IDs: PRJNA907518
Additional info:
 
Sample processing
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
463 2018-01-22 4137 (23.1.2018) 992.0 (QUBIT) 2 mm3 60 29668.0 QIAGEN RNeasy Mini Kit 10.00 2.08 1.84
 
Applied assays   
STRT2-seq
 
Technical replicate [1014]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP3_32 Illumina NextSeq 550 No Dec. 12, 2018 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP3_32  STRT2_FP3_32_canine_mapped.bam STRT2_FP3_32_canine_mapped.fq.gz CanFam3.1 3.0
STRT2_FP3_32  STRT2_FP3_32_canine_mapped.fq.gz CanFam3.1 3.0
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP3_32 219,392 5,642,057 70.90