Technical replicate [1375]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP5_44	Illumina	NextSeq 550	No	June 14, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP5_44  STRT2_FP5_44_canine_mapped.bam STRT2_FP5_44_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP5_44  STRT2_FP5_44_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP5_44 — — 796 — 7,200 66.60 — —

Technical replicate [1096]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP5_41	Illumina	NextSeq 550	No	June 14, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP5_41  STRT2_FP5_41_canine_mapped.bam STRT2_FP5_41_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP5_41  STRT2_FP5_41_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP5_41 — — 124,949 — 1,712,456 65.30 — —

Technical replicate [1049]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP3_37	Illumina	NextSeq 550	No	Dec. 12, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP3_37  STRT2_FP3_37_canine_mapped.bam STRT2_FP3_37_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP3_37  STRT2_FP3_37_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP3_37 — — 193,330 — 2,906,329 70.90 — —