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Sample description
Sample ID: 4746
Anatomical region: abdominal cavity
Organ system: reproductive system
Term: ovary
Sub-term: right ovary
Sampling: 2018-02-07 / provided by Lohi lab
Transport media: RNAlater
Additional info: Not all the tissues were collected from this dog.
From subject: VEO010
Breed (variant): East-European Shepherd
Sex: Female
Age: 2y 10m
Steril./Castr.: Not specified
Reports:  Necropsy report 
WGS ID:
Additional info:
Study: Study of gene expression across dog tissues
Description: This project will generate the critical resources to better utilize various canine models to advance both dog and human health.
Status / Visibility: Submitted / Public
Publication: The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728)
Other IDs: PRJNA907518
Additional info:
 
Sample processing
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
799 2019-02-19 4746 (25.2.2019) 307.0 (QUBIT) 2 mm3 40 9080.0 QIAGEN RNeasy Mini Kit 9.70 2.04 1.77
 
Applied assays   
STRT2-seq
 
Technical replicate [1507]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP13_43 Illumina NextSeq 550 No Aug. 14, 2019 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP13_43  SDID_001883_STRT2_FP13_43_canine_mapped.bam SDID_001882_STRT2_FP13_43_canine_mapped.fq.gz CanFam3.1 3.0
STRT2_FP13_43  SDID_001882_STRT2_FP13_43_canine_mapped.fq.gz CanFam3.1 3.0
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP13_43 134,549 1,418,369 70.80