Technical replicate [1361]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP11_30	Illumina	NextSeq 550	No	Oct. 25, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP11_30  STRT2_FP11_30_canine_mapped.bam STRT2_FP11_30_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP11_30  STRT2_FP11_30_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP11_30 — — 187,181 — 2,992,840 75.70 — —

Technical replicate [958]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP2_3	Illumina	NextSeq 550	No	Nov. 27, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP2_3  STRT2_FP2_3_canine_mapped.bam STRT2_FP2_3_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP2_3  STRT2_FP2_3_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP2_3 — — 395 — 9,247 62.80 — —

Technical replicate [933]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP1_30	Illumina	NextSeq 550	No	April 19, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP1_30  STRT2_FP1_30_canine_mapped.bam STRT2_FP1_30_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP1_30  STRT2_FP1_30_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP1_30 — — 227,590 — 1,867,816 58.60 — —