Technical replicate [1359]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP11_29	Illumina	NextSeq 550	No	Oct. 25, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP11_29  STRT2_FP11_29_canine_mapped.bam STRT2_FP11_29_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP11_29  STRT2_FP11_29_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP11_29 — — 173,320 — 3,004,331 77.30 — —

Technical replicate [999]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP2_2	Illumina	NextSeq 550	No	Nov. 27, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP2_2  STRT2_FP2_2_canine_mapped.bam STRT2_FP2_2_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP2_2  STRT2_FP2_2_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP2_2 — — 104,064 — 1,776,580 67.20 — —

Technical replicate [930]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP1_29	Illumina	NextSeq 550	No	April 19, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP1_29  STRT2_FP1_29_canine_mapped.bam STRT2_FP1_29_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP1_29  STRT2_FP1_29_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP1_29 — — 145,601 — 1,033,500 60.00 — —