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Sample description
Sample ID: 515
Anatomical region: head
Organ system: central nervous system
Term: amygdala
Sub-term: amygdala
Sampling: 2017-03-06 / provided by Lohi lab
Transport media: RNAlater
Additional info: List of tissues was modified from previous necropsy.
From subject: JPK344
Breed (variant): Swedish Elkhound
Sex: Female
Age: 3y 5m
Steril./Castr.: No
Reports:  Necropsy report   Clinical test results
WGS ID: JPK344A1, BioProject PRJNA907518, SRR22520748
Additional info: Two generalized epileptic seizures.
Study: Study of gene expression across dog tissues
Description: This project will generate the critical resources to better utilize various canine models to advance both dog and human health.
Status / Visibility: Submitted / Public
Publication: The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728)
Other IDs: PRJNA907518
Additional info:
 
Sample processing
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
331 2017-05-17 JPK344 515 100.0 (ND) 19 mg 30 3000.0 QIAGEN RNeasy Mini Kit 8.00 2.059 0.612
 
Applied assays   
CAGE-seq
 
Technical replicate [1749]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
CAGE_L_00201.BC05 Illumina HiSeq 2500 No Aug. 20, 2020 SR 1x50bp 50 bp HiSeq SR Rapid Cluster Kit v2 GD-402-4002 + HiSeq Rapid Duo cBot Sample Loading Kit CT-403-2001 + SBS Clinical Genomics Stockholm
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
CAGE_L_00201.BC05  CAGE_L_00201.BC05_qfilter_trimBC.fq.gz 4
CAGE_L_00201.BC05  CAGE_L_00201.BC05.sorted.tar.gz CAGE_L_00201.BC05_qfilter_trimBC.fq.gz CanFam4 4
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
CAGE_L_00201.BC05 caudate nucleus- amygdala pool 86,146,533 4,720,329 65.30