Technical replicate [947]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP1_40	Illumina	NextSeq 550	No	April 19, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP1_40  STRT2_FP1_40_canine_mapped.bam STRT2_FP1_40_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP1_40  STRT2_FP1_40_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP1_40 — — 212,381 — 19,290 5.50 — —

Technical replicate [1387]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP11_40	Illumina	NextSeq 550	No	Oct. 25, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP11_40  STRT2_FP11_40_canine_mapped.bam STRT2_FP11_40_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP11_40  STRT2_FP11_40_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP11_40 — — 166,364 — 2,685,422 77.70 — —

Technical replicate [1350]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP11_18	Illumina	NextSeq 550	No	Oct. 25, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP11_18  STRT2_FP11_18_canine_mapped.bam STRT2_FP11_18_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP11_18  STRT2_FP11_18_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP11_18 — — 236,311 — 1,741,666 73.50 — —