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Sample description
Sample ID: 545
Anatomical region: head
Organ system: central nervous system
Term: pons
Sub-term: pons
Sampling: 2017-03-06 / provided by Lohi lab
Transport media: RNAlater
Additional info: List of tissues was modified from previous necropsy.
From subject: JPK344
Breed (variant): Swedish Elkhound
Sex: Female
Age: 3y 5m
Steril./Castr.: No
Reports:  Necropsy report   Clinical test results
WGS ID: JPK344A1, BioProject PRJNA907518, SRR22520748
Additional info: Two generalized epileptic seizures.
Study: Study of gene expression across dog tissues
Description: This project will generate the critical resources to better utilize various canine models to advance both dog and human health.
Status / Visibility: Submitted / Public
Publication: The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728)
Other IDs: PRJNA907518
Additional info:
 
Sample processing
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
670 2017-08-16 545 (15.11.2018) 70 (ND) 17 mg 30 2100 QIAZOL + QIAGEN RNeasy Mini Kit 9.00 2.043 2.036
 
Applied assays   
STRT2-seq
 
Technical replicate [1364]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP11_33 Illumina NextSeq 550 No Oct. 25, 2018 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP11_33  STRT2_FP11_33_canine_mapped.bam STRT2_FP11_33_canine_mapped.fq.gz CanFam3.1 3.0
STRT2_FP11_33  STRT2_FP11_33_canine_mapped.fq.gz CanFam3.1 3.0
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP11_33 250,186 1,567,744 71.90
Technical replicate [956]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP1_33 Illumina NextSeq 550 No April 19, 2018 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP1_33  STRT2_FP1_33_canine_mapped.bam STRT2_FP1_33_canine_mapped.fq.gz CanFam3.1 3.0
STRT2_FP1_33  STRT2_FP1_33_canine_mapped.fq.gz CanFam3.1 3.0
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP1_33 156,535 508,003 52.60
 
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
144 2017-04-12 545 37.0 (QUBIT) 16 mg 30 1110.0 QIAGEN RNeasy Mini Kit 8.70 2.063 0.557
 
Applied assays   
This sample was not sequenced yet.
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
313 2017-07-15 JPK344 545 70.0 (ND) 17 mg 30 2100.0 Not specified 9.00 2.043 2.036
 
Applied assays   
This sample was not sequenced yet.