Technical replicate [1927]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
CAGE_L_00216.BC05	Illumina	HiSeq 2500	No	Nov. 27, 2020	SR 1x50bp	50 bp	HiSeq SR Rapid Cluster Kit v2 GD-402-4002 + HiSeq Rapid Duo cBot Sample Loading Kit CT-403-2001 + SBS	Clinical Genomics Stockholm
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments CAGE_L_00216.BC05  CAGE_L_00216.BC05.sorted.tar.gz CAGE_L_00216.BC05_qfilter_trimBC.fq.gz CanFam4 — 4 — CAGE_L_00216.BC05  CAGE_L_00216.BC05_qfilter_trimBC.fq.gz — — — 4 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments CAGE_L_00216.BC05 Brain stem pool 98,666,299 — — 3,715,121 60.68 — —

Technical replicate [1364]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP11_33	Illumina	NextSeq 550	No	Oct. 25, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP11_33  STRT2_FP11_33_canine_mapped.bam STRT2_FP11_33_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP11_33  STRT2_FP11_33_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP11_33 — — 250,186 — 1,567,744 71.90 — —

Technical replicate [956]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP1_33	Illumina	NextSeq 550	No	April 19, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP1_33  STRT2_FP1_33_canine_mapped.bam STRT2_FP1_33_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP1_33  STRT2_FP1_33_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP1_33 — — 156,535 — 508,003 52.60 — —