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Sample description
Sample ID: 5704
Anatomical region: head
Organ system: central nervous system
Term: corpus callosum
Sub-term: corpus callosum
Sampling: — / provided by Gusev's Lab
Transport media: Not specified
Additional info: Wolf (I) from Russia
From subject: WLF019
Breed (variant): Wolf
Sex: Male
Age: Not specified
Steril./Castr.: Not specified
Reports:
WGS ID:
Additional info: Wolf (I) from Russia
Study: Study of gene expression across dog tissues
Description: This project will generate the critical resources to better utilize various canine models to advance both dog and human health.
Status / Visibility: Submitted / Public
Publication: The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728)
Other IDs: PRJNA907518
Additional info:
 
Sample processing
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
596 2018-06-19 corpus callosum 156 (QUBIT) 50 4958 QIAGEN RNeasy Mini Kit 7.30 2.159 2.169
 
Applied assays   
This sample was not sequenced yet.
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
720 2018-06-15 corpus callosum 36.28 (ND) 50 1814.0 QIAGEN RNeasy Mini Kit 8.00 1.84 2.223
 
Applied assays   
STRT2-seq
 
Technical replicate [1288]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP10_9 Illumina NextSeq 550 No Oct. 12, 2018 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP10_9  STRT2_FP10_9_canine_mapped.bam STRT2_FP10_9_canine_mapped.fq.gz CanFam3.1 3.0
STRT2_FP10_9  STRT2_FP10_9_canine_mapped.fq.gz CanFam3.1 3.0
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP10_9 337,554 1,937,798 65.00