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Sample description
Sample ID: 5724
Anatomical region: head
Organ system: central nervous system
Term: cerebellum hemisphere
Sub-term: cerebellum hemisphere (left side)
Sampling: — / provided by Gusev's Lab
Transport media: Not specified
Additional info: Wolf (II) from Russia
From subject: WLF020
Breed (variant): Wolf
Sex: Male
Age: Not specified
Steril./Castr.: Not specified
Reports: —
WGS ID: —
Additional info: Wolf (II) from Russia
Study: Study of gene expression across dog tissues
Description: This project will generate the critical resources to better utilize various canine models to advance both dog and human health.
Status / Visibility: Submitted / Public
Publication: The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728)
Other IDs: PRJNA907518
Additional info: —
 
Sample processing
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)		 Tissue ammount Sample volume RNA amount
(ng)		 Extraction method RIN Ratio
A260/A280		 Ratio
A260/A230		 Comments
576 2018-06-01 RNeasy Mini 59.0 (ND) — 50 2964.0 QIAGEN RNeasy Mini Kit 7.10 2.093 2.327 —
 
Applied assays   
STRT2-seq
 
Technical replicate [1604]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP15_14 Illumina NextSeq 550 No Aug. 15, 2019 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP15_14  SDID_002001_STRT2_FP15_14_canine_mapped.bam SDID_002000_STRT2_FP15_14_canine_mapped.fq.gz CanFam3.1 — 3.0 —
STRT2_FP15_14  SDID_002000_STRT2_FP15_14_canine_mapped.fq.gz — CanFam3.1 — 3.0 —
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP15_14 — — 136,990 — 1,482,980 65.80 — —