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Sample description
Sample ID: 5725
Anatomical region: head
Organ system: central nervous system
Term: cerebellum vermis
Sub-term: cerebellum vermis
Sampling: — / provided by Gusev's Lab
Transport media: Not specified
Additional info: Wolf (II) from Russia
From subject: WLF020
Breed (variant): Wolf
Sex: Male
Age: Not specified
Steril./Castr.: Not specified
Reports: —
WGS ID: —
Additional info: Wolf (II) from Russia
Study: Study of gene expression across dog tissues
Description: This project will generate the critical resources to better utilize various canine models to advance both dog and human health.
Status / Visibility: Submitted / Public
Publication: The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728)
Other IDs: PRJNA907518
Additional info: —
 
Sample processing
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)		 Tissue ammount Sample volume RNA amount
(ng)		 Extraction method RIN Ratio
A260/A280		 Ratio
A260/A230		 Comments
575 2018-06-13 RNeasy Mini 53.0 (ND) — 50 2684.0 QIAGEN RNeasy Mini Kit 7.30 1.971 3.128 —
 
Applied assays   
STRT2-seq
 
Technical replicate [1601]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP15_13 Illumina NextSeq 550 No Aug. 15, 2019 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP15_13  SDID_001999_STRT2_FP15_13_canine_mapped.bam SDID_001998_STRT2_FP15_13_canine_mapped.fq.gz CanFam3.1 — 3.0 —
STRT2_FP15_13  SDID_001998_STRT2_FP15_13_canine_mapped.fq.gz — CanFam3.1 — 3.0 —
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP15_13 — — 166,357 — 1,195,704 63.60 — —