Sample ID: | 5740 |
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Anatomical region: | forelimb |
Organ system: | integumental system |
Term: | skin |
Sub-term: | skin (fibroblast) |
Sampling: | 2017-04-11 / provided by Lohi lab |
Transport media: | Not specified |
Additional info: | — |
From subject: | RW356 |
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Breed (variant): | Rottweiler |
Sex: | Male |
Age: | 6y 2m |
Steril./Castr.: | No |
Reports: | Necropsy report Clinical test results |
WGS ID: | RW356A1, BioProject PRJNA907518, SRR22520747 |
Additional info: | — |
Study: | Study of gene expression across dog tissues |
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Description: | This project will generate the critical resources to better utilize various canine models to advance both dog and human health. |
Status / Visibility: | Submitted / Public |
Publication: | The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728) |
Other IDs: | PRJNA907518 |
Additional info: | — |
RNA extraction | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ID | Date | Bioanalizer ID | Stock Conc. (ng/µl) |
Tissue ammount | Sample volume | RNA amount (ng) |
Extraction method | RIN | Ratio A260/A280 |
Ratio A260/A230 |
Comments | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
630 | 2018-04-05 | RW356 RNA T4 (8.6.2018) | 281.0 (ND) | — | 100 | 28136.0 | QIAGEN RNeasy Mini Kit | 10.00 | 2.08 | 1.72 | Amount of cells: 10 cm PD 70% confluence. Same elution volume going twice through the column | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applied assays
STRT2-seq
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