| Sample ID: | 5740 |
|---|---|
| Anatomical region: | forelimb |
| Organ system: | integumental system |
| Term: | skin |
| Sub-term: | skin (fibroblast) |
| Sampling: | 2017-04-11 / provided by Lohi lab |
| Transport media: | Not specified |
| Additional info: | — |
| From subject: | RW356 |
|---|---|
| Breed (variant): | Rottweiler |
| Sex: | Male |
| Age: | 6y 2m |
| Steril./Castr.: | No |
| Reports: | Necropsy report Clinical test results |
| WGS ID: | RW356A1, BioProject PRJNA907518, SRR22520747 |
| Additional info: | — |
| Study: | Study of gene expression across dog tissues |
|---|---|
| Description: | This project will generate the critical resources to better utilize various canine models to advance both dog and human health. |
| Status / Visibility: | Submitted / Public |
| Publication: | The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728) |
| Other IDs: | PRJNA907518 |
| Additional info: | — |
| RNA extraction | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| ID | Date | Bioanalizer ID | Stock Conc. (ng/µl) |
Tissue ammount | Sample volume | RNA amount (ng) |
Extraction method | RIN | Ratio A260/A280 |
Ratio A260/A230 |
Comments | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 630 | 2018-04-05 | RW356 RNA T4 (8.6.2018) | 281.0 (ND) | — | 100 | 28136.0 | QIAGEN RNeasy Mini Kit | 10.00 | 2.08 | 1.72 | Amount of cells: 10 cm PD 70% confluence. Same elution volume going twice through the column | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Applied assays
STRT2-seq
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