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Sample description
Sample ID: 59
Anatomical region: head
Organ system: central nervous system
Term: midbrain
Sub-term: substantia nigra
Sampling: 2017-02-16 / provided by Lohi lab
Transport media: RNAlater
Additional info: Dissection date 16.2.2017
From subject: SLK1048
Breed (variant): Finnish Lapphund
Sex: Female
Age: 6y 6m
Steril./Castr.: Yes
Reports:  Necropsy report   Clinical test results
WGS ID: SLK1048A1, BioProject PRJNA907518, SRR22520749
Additional info:
Study: Study of gene expression across dog tissues
Description: This project will generate the critical resources to better utilize various canine models to advance both dog and human health.
Status / Visibility: Submitted / Public
Publication: The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728)
Other IDs: PRJNA907518
Additional info:
 
Sample processing
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
84 2017-03-06 59 (13.3.2017) 39.0 (QUBIT) 30 mg 40 1600.0 QIAZOL + QIAGEN RNeasy Mini Kit 8.90 2.016 0.29
 
Applied assays   
STRT2-seq
 
Technical replicate [909]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP1_11 Illumina NextSeq 550 No April 19, 2018 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP1_11  STRT2_FP1_11_canine_mapped.bam STRT2_FP1_11_canine_mapped.fq.gz CanFam3.1 3.0
STRT2_FP1_11  STRT2_FP1_11_canine_mapped.fq.gz CanFam3.1 3.0
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP1_11 3,595 27,253 34.60
Technical replicate [1342]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP11_11 Illumina NextSeq 550 No Oct. 25, 2018 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP11_11  STRT2_FP11_11_canine_mapped.bam STRT2_FP11_11_canine_mapped.fq.gz CanFam3.1 3.0
STRT2_FP11_11  STRT2_FP11_11_canine_mapped.fq.gz CanFam3.1 3.0
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP11_11 214,957 1,903,479 72.30