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Sample description
Sample ID: 625
Anatomical region: head
Organ system: eye
Term: retina
Sub-term: retina
Sampling: 2017-03-06 / provided by Lohi lab
Transport media: RNAlater
Additional info: List of tissues was modified from previous necropsy.
From subject: JPK344
Breed (variant): Swedish Elkhound
Sex: Female
Age: 3y 5m
Steril./Castr.: No
Reports:  Necropsy report   Clinical test results
WGS ID: JPK344A1, BioProject PRJNA907518, SRR22520748
Additional info: Two generalized epileptic seizures.
Study: Study of gene expression across dog tissues
Description: This project will generate the critical resources to better utilize various canine models to advance both dog and human health.
Status / Visibility: Submitted / Public
Publication: The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728)
Other IDs: PRJNA907518
Additional info:
 
Sample processing
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
117 2018-11-12 625 (15.11.2018) 736.0 (ND) 2 mm3 42 30914.0 QIAZOL + QIAGEN RNeasy Mini Kit 9.00 2.11 1.79 Pooled tissues 625&626 because the pieces were too little. Ceramic bead homogenization.
 
Applied assays   
CAGE-seq
 
Technical replicate [1887]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
CAGE_L_00212.BC15 Illumina HiSeq 2500 No Oct. 6, 2020 SR 1x50bp 50 bp HiSeq SR Rapid Cluster Kit v2 GD-402-4002 + HiSeq Rapid Duo cBot Sample Loading Kit CT-403-2001 + SBS Clinical Genomics Stockholm
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
CAGE_L_00212.BC15  CAGE_L_00212.BC15_qfilter_trimBC.fq.gz 4
CAGE_L_00212.BC15  CAGE_L_00212.BC15.sorted.tar.gz CAGE_L_00212.BC15_qfilter_trimBC.fq.gz CanFam4 4
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
No quality data available.