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Sample description
Sample ID: 6608
Anatomical region: all parts
Organ system: all systems
Term: embryo
Sub-term: embryo
Sampling: — / provided by Werch’s Lab
Transport media: Liquid nitrogen
Additional info:
From subject: BD410-2
Breed (variant): Mixed Breed
Sex: Not specified
Age: Not specified
Steril./Castr.: Not specified
Reports:
WGS ID:
Additional info: Embryo. Age about 30 days.
Study: Study of gene expression across dog tissues
Description: This project will generate the critical resources to better utilize various canine models to advance both dog and human health.
Status / Visibility: Submitted / Public
Publication: The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728)
Other IDs: PRJNA907518
Additional info:
 
Sample processing
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
863 2019-11-29 BD410 #2dil.(TS 5.12.2019) 103.0 (ND) 50 5176.0 QIAGEN RNeasy Mini Kit 9.50 1.88 1.76
 
Applied assays   
CAGE-seq
 
Technical replicate [1815]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
CAGE_L_00207.BC05 Illumina HiSeq 2500 No Sept. 29, 2020 SR 1x50bp 50 bp HiSeq SR Rapid Cluster Kit v2 GD-402-4002 + HiSeq Rapid Duo cBot Sample Loading Kit CT-403-2001 + SBS Clinical Genomics Stockholm
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
CAGE_L_00207.BC05  CAGE_L_00207.BC05_qfilter_trimBC.fq.gz 4
CAGE_L_00207.BC05  CAGE_L_00207.BC05.sorted.tar.gz CAGE_L_00207.BC05_qfilter_trimBC.fq.gz CanFam4 4
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
CAGE_L_00207.BC05 Retina 89,460,328 5,799,082 58.35
 
STRT2-seq
 
Technical replicate [1674]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
STRT2_FP16_33 Illumina NextSeq 550 No Dec. 23, 2019 SR 1x75bp 75bp NextSeq High Output FuGU lab Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
STRT2_FP16_33  SDID_002152_STRT2_FP16_33_canine_mapped.bam SDID_002151_STRT2_FP16_33_canine_mapped.fq.gz CanFam3.1 3.0
STRT2_FP16_33  SDID_002151_STRT2_FP16_33_canine_mapped.fq.gz CanFam3.1 3.0
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
STRT2_FP16_33 18,674 3.80