Technical replicate [1576]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP14_4	Illumina	NextSeq 550	No	May 2, 2019	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP14_4  SDID_001980_STRT2_FP14_4_canine_mapped.fq.gz — CanFam3.1 — 3.0 — STRT2_FP14_4  SDID_001981_STRT2_FP14_4_canine_mapped.bam SDID_001980_STRT2_FP14_4_canine_mapped.fq.gz CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP14_4 — — 113,190 — 5,109,995 73.80 — —

Technical replicate [1179]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP7_4	Illumina	NextSeq 550	No	Aug. 16, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP7_4  STRT2_FP7_4_canine_mapped.bam STRT2_FP7_4_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP7_4  STRT2_FP7_4_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP7_4 — — — — 1,470,808 70.20 — —

Technical replicate [1743]
Other ID	Platform	Instrument	Paired end	Seq. date	Strand mode	Max. read length	Chemistry version	Laboratory	Comments
STRT2_FP7_43	Illumina	NextSeq 550	No	Dec. 12, 2018	SR 1x75bp	75bp	NextSeq High Output	FuGU lab	Phi-X spike in added to each STRT2 library for sequencing (20%).
Sequencing information Sequencing data Seq. ID Files Derived from Mapped genome Command Freeze version Comments STRT2_FP7_43  STRT2_FP7_43_canine_mapped.bam STRT2_FP7_43_canine_mapped.fq.gz CanFam3.1 — 3.0 — STRT2_FP7_43  STRT2_FP7_43_canine_mapped.fq.gz — CanFam3.1 — 3.0 —   Sequencing quality data Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments STRT2_FP7_43 — — — — 2,680,220 77.00 — —