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Sample description
Sample ID: 795
Anatomical region: abdominal cavity
Organ system: alimentary system
Term: liver
Sub-term: liver left lobe
Sampling: 2017-03-06 / provided by Lohi lab
Transport media: RNAlater
Additional info: List of tissues was modified from previous necropsy.
From subject: JPK344
Breed (variant): Swedish Elkhound
Sex: Female
Age: 3y 5m
Steril./Castr.: No
Reports:  Necropsy report   Clinical test results
WGS ID: JPK344A1, BioProject PRJNA907518, SRR22520748
Additional info: Two generalized epileptic seizures.
Study: Study of gene expression across dog tissues
Description: This project will generate the critical resources to better utilize various canine models to advance both dog and human health.
Status / Visibility: Submitted / Public
Publication: The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nat. Commun. 2024, 15(1):9082 (PMID: 39433728)
Other IDs: PRJNA907518
Additional info:
 
Sample processing
RNA extraction
ID Date Bioanalizer ID Stock Conc.
(ng/µl)
Tissue ammount Sample volume RNA amount
(ng)
Extraction method RIN Ratio
A260/A280
Ratio
A260/A230
Comments
643 2018-11-22 795 (23.11.2018) 230.0 (QUBIT) 3 mm3 100 17683.0 QIAGEN RNeasy Mini Kit 9.80 2.07 1.11 Increased amount of ETOH (75%) instead the 70% recommended by manufacturer
 
Applied assays   
CAGE-seq
 
Technical replicate [1803]
Other ID Platform Instrument Paired end Seq. date Strand mode Max. read length Chemistry version Laboratory Comments
CAGE_L_00205.BC13 Illumina HiSeq 2500 No Sept. 23, 2020 SR 1x50bp 50 bp HiSeq SR Rapid Cluster Kit v2 GD-402-4002 + HiSeq Rapid Duo cBot Sample Loading Kit CT-403-2001 + SBS Clinical Genomics Stockholm
Sequencing information
Sequencing data
Seq. ID Files Derived from Mapped genome Command Freeze version Comments
CAGE_L_00205.BC13  CAGE_L_00205.BC13_qfilter_trimBC.fq.gz 4
CAGE_L_00205.BC13  CAGE_L_00205.BC13.sorted.tar.gz CAGE_L_00205.BC13_qfilter_trimBC.fq.gz CanFam4 4
 
Sequencing quality data
Other ID Pool name BC reads Spike-in mapped reads Spike-in mapped % Canine mapped reads Canine mapped % Unmapped reads % Comments
CAGE_L_00205.BC13 Liver left lope only 110,145,906 7,868,308 56.53